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| 产品名称： | LOVO |
|---|---|
| 商品货号： | TS259449 |
| <strong>Cell line </strong>： | LOVO |
| DSMZ no. ： | TS259449 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the metastatic nodule resected from a 56-year-old Caucasian man with colon adenocarcinoma in 1972; cells were described in the literature to produce CEA |
| Reference(s) ： | 14468, 14469, 14470 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 35 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 16% polyploidy - 48/49<2n>XY, +7, +1-2mar, t(2;12)(q21;p11) - +7 recurrent in colon carcinoma - 1-2 small satellited markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |