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| 产品名称： | RTG-2 |
|---|---|
| 商品货号： | TS259450 |
| <strong>Cell line </strong>： | RTG-2 |
| DSMZ no. ： | TS259450 |
| Species ： | originally confirmed as trout spec. by species-PCR and later as Oncorhynchus mykiss origin by COI DNA Barcoding |
| Cell type ： | gonadal cells |
| Origin ： | established from the gonadal cells of a rainbow trout (salmo gairdneri) |
| Reference(s) ： | 15067 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | long spindle-like and fibroblast-like cells growing adherently in monolayers |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 2 mM L-glutamine + 50 mg/L neomycin sulfate |
| Subculture ： | split subconfluent culture 1:2 every 5-7 days using trypsin/EDTA at cold temperature; seed out at ca. 0.5 x 106 cells/25 cm2; growth is inhibited at a pH higher than 7.4 |
| Incubation ： | maximum temperature at 26 °C, optimal at 20 °C, grown without CO2 (keep lid of flask closed) |
| Doubling time ： | ca. 3-7 days |
| Harvest ： | cell harvest of ca. 4 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization and PCR assays |
| Viruses ： | PCR: SMRV - |