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| 产品名称： | KYSE-30 |
|---|---|
| 商品货号： | TS259454 |
| <strong>Cell line </strong>： | KYSE-30 |
| DSMZ no. ： | TS259454 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line established from tumor cells heterotransplanted into athymic mice; described in the literature to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1 |
| Reference(s) ： | 14955, 15006, 14646, 14954 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid with long processes growing in monolayers; image; image ; image |
| Medium ： | 45% RPMI 1640 + 45% Hams F12 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-4 x 106 cells/175 cm2; split confluent culture 1:3 to 1:10 every 2-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 0.7 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | highly rearranged hypertriploid karyotype with 8% polyploidy - 66-74<3n>Xder(X), -Y, +1, +1, -5, +7, -9, -10, -15, +16, -18, -18, -19, -21, -21, -21, -22, -22, +12-15mar, t(X;2)(p11;p11), ins(1;1)(p34/36;q21q32), add(1)(q11), der(1)add(1) (p21)(q22), del(2) (q24), der(2)t(2;4)(p11;q11)t(2;4)(q37;q11), del(3)(p14), add(3)(p11), add(4)(q11)x2, der(4)t(1;4)(p11;q11), add(5)(q11), add(5)(q11), add(6)(p21), i(7q), del(7)(p14), add(7)(p14), der(8)t(7;8)(q11;p11), add(9)(p24), i(10q), del(11)(q11)x1-2, add(11)(p13), del(12) (p12.2), i(13q), add(14)(q32), add(15)(p11), add(16)(p1?), add(17)(p1?), add(19)(q13.1)x2, inc. - extreme level of rearrangement |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |