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| 产品名称： | SU-DHL-1 |
|---|---|
| 商品货号： | TS259460 |
| <strong>Cell line </strong>： | SU-DHL-1 |
| DSMZ no. ： | TS259460 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and recently by STR-typing |
| Cell type ： | anaplastic large cell lymphoma |
| Origin ： | established from the pleural effusion of a 10-year-old boy in 1973; original diagnosis was diffuse histiocytic lymphoma, corrected to diffuse large cell lymphoma (1984) and malignant histiocytosis (1989); according to REAL/WHO classification considered to represent an anaplastic large cell lymphoma (ALCL); described in the literature to carry the t(2;5)(p23;q35) NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14515, 14737, 14513, 14492, 14464, 14516, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | polymorphic cells growing in suspension, singly or in small clusters; image; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days; culture may contain a certain amount of debris (which can be removed by centrifugation) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD5 +, CD10 -, CD13 -, CD19 -, CD20 -, CD25 +, CD30 +, CD34 -, CD37 -, CD38 -, CD71 +, CD80 -, CD138 -, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 16% polyploidy - 74(67-75)<3n>XX, -Y, +1, +2, +3, +5, -7, +12, -16, -18, +19, -20, +21, +3mar, del(1)(p21), t(2;5)(p23;q35)x2, del(6)(q23)x1-2, add(8)(p12), add(9)(p21), del(10)(p14), add(12)(q24), add(14)(p12), add(16)(q24), dup(19)(q13.1qter) - sideline with der(12)t(12;19)(q13;q13) - carries two copies of balanced t(2;5) associated with ALCL - matches published karyotype - expression of NPM1-ALK (NPM-ALK) fusion gene was detected by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |