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| 产品名称： | HCT-15 |
|---|---|
| 商品货号： | TS259461 |
| <strong>Cell line </strong>： | HCT-15 |
| DSMZ no. ： | TS259461 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MPI, later by STR typing |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from a colorectal adenocarcinoma; HCT-15 is a sister cell line of the cell line DLD-1 (DSM ACC 278) |
| Reference(s) ： | 14456, 15218 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer with contact inhibition; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out 2 x 106 cells in a 80 cm2 flask; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA; maximal density at ca. 50 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-25 hours |
| Harvest ： | harvest of ca. 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as DLD-1 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human pseudodiploid karyotype with 16% polyploidy - 46<2n>XYq+, t(8;17) (p23;q12/21), inv(11)(p14.2;q13) - stable karyotypic differences cf DLD-1 - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |