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| 产品名称： | MHH-PREB-1 |
|---|---|
| 商品货号： | TS259467 |
| <strong>Cell line </strong>： | MHH-PREB-1 |
| DSMZ no. ： | TS259467 |
| Species ： | confirmed as human with IEF of AST, LDH, MPI, PEP B |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the lymph node of a 5-year-old Caucasian boy with B cell lymphoblastic non-Hodgkin lymphoma at diagnosis in 1994 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in clusters in suspension; image; image |
| Medium ： | 90-92.5% RPMI 1640 + 7.5-10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 105 cells/ml; maintain at 0.5-2.0 x 106 cells/ml; split 1:10 to 1:20 twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-40 hours |
| Harvest ： | cell harvest of ca. 2.5 x 106/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 48<2n>XYY, +21, ?ins(2;2)(q21;p15p22), t(8;14)(q24;q32) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |