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| 产品名称： | MOLT-4 |
|---|---|
| 商品货号： | TS259469 |
| <strong>Cell line </strong>： | MOLT-4 |
| DSMZ no. ： | TS259469 |
| Species ： | confirmed as human originally with IEF of AST, LDH, NP, later by STR typing |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 19-year-old man with acute lymphoblastic leukemia (ALL) in relapse in 1971; sister cell line of MOLT-3 (DSMZ ACC 84). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14767, 15217, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in suspension, singly or in clusters; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-1.3 x 106 cells/ml; split ratio of ca. 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximal density at ca. 1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 +, cyCD3 +, smCD3 -, CD5 +, CD6 +, CD7 +, CD13 -, CD19 -, CD34 +, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | same DNA profile as MOLT-3 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype - 89-99<4n>XXYY, +4, +7, +8, +20, +20, del(6)(q16)x2, der(7)t(7;7)(p15;q11)x2 - closely related to MOLT-3 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |