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| 产品名称： | CGTH-W-1 |
|---|---|
| 商品货号： | TS259471 |
| <strong>Cell line </strong>： | CGTH-W-1 |
| DSMZ no. ： | TS259471 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | thyroid carcinoma (derivative of SW-579) |
| Origin ： | established from the sternal metastasis of follicular thyroid carcinoma removed from a 70-year-old Chinese woman in 1993; described in the literature to carry IGF-1 receptors; DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of human thyroid carcinoma cell line SW-579 |
| Reference(s) ： | 15149 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent large epitheloid cells with long processes growing in monolayers with multilayer foci |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:10 every 2-5 days using trypsin/EDTA; seed out at ca. 1-4 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 20-25 hours |
| Harvest ： | cell harvest of ca. 30-50 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 10% polyploidy - 77(72-78)<3n>XX, -X, +1, +3, +3, +6, +8, +16, -17, -21, +5mar, del(X)(q25)x2, del(1)(q22), t(3;10)(p11;q11), ?del(4) (q21), del(6)(p11p21), del(7)(p14), i(9q), ?del(10)(q21/24), der(11)t(11;14)(q24;q12), del(13)(q31)x2, add(15)(q25)x2, del(17)(p11), ?add(18)(p11) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |