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| 产品名称： | BFTC-905 |
|---|---|
| 商品货号： | TS259472 |
| <strong>Cell line </strong>： | BFTC-905 |
| DSMZ no. ： | TS259472 |
| Species ： | confirmed as human with IEF of AST, LDH |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from a 51-year-old Chinese woman with a grade III papillary transitional cell carinoma of the urinary bladder in 1990; patient lived in a blackfoot disease endemic area in Taiwan |
| Reference(s) ： | 14430, 15028, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers, forming large islets; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split culture before reaching confluency 1:3 to 1:5 once or twice a week using trypsin/EDTA (for 5-10 min) |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 60-70 hours |
| Harvest ： | cell harvest of ca. 10-15 x 10 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS and 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays; bacterial contamination was eliminated with Baytril (enrofloxacin) and BM-Cyclin (tiamulin & minocycline) |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hyperdiploid/hypotriploid karyotype - 58(52-61)<3n>X/XX-X, -4, +5, -9, -13, -15, -16, -18, -21, -22, -22, +mar, add(1)(p36), i(5p), add(5)(q35), del(9)(p22), del(11)(p11), add(11)(q13), add(13)(p12), add(13)(p12), add(15)(p12), add(19)(p13) - i(5p) and loss of 9p associated with transitional cell carcinoma - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |