微信小李
微信小章
产品简介
购买须知
| 产品名称： | DBTRG-05MG |
|---|---|
| 商品货号： | TS259475 |
| <strong>Cell line </strong>： | DBTRG-05MG |
| DSMZ no. ： | TS259475 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | glioblastoma |
| Origin ： | established from the primary tumor cells of a 59-year-old Caucasian woman with glioblastoma multiforme following irradiation and chemotherapy in 1985; cells were described in the literature to express NSE (neuron-specific enolase) and EGF-R (epidermal growth factor receptor) |
| Reference(s) ： | 14688 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle, fibroblast-like cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 12% polyploidy - 81-88<4n>XXX, -X, -1, -1, -3, -4, -4, +7, +7, -10, -10, -13, -13, -14, -14, -16, +6mar, der(4)t(4;14)(p11;q11), add(5)(p15)x2, add(7)(p22)x3, del(8)(q22), inv(9)(p13q34)x1-2, add(11)(p15)x2, der(11)t(1;11)(q23;q21), del(11)(q13), add(16)(q24)x2 - +7 and -10 recurrent in gliomas - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |