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| 产品名称： | HH-16.cl.4 |
|---|---|
| 商品货号： | TS259476 |
| <strong>Cell line </strong>： | HH-16.cl.4 |
| DSMZ no. ： | TS259476 |
| Species ： | originally confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | mammary tumor |
| Origin ： | established from the ascitic fluid of a Sprague-Dawley rat (adult female, 240-days-old) with a rat mammary tumor in 1977; tumor was produced by injection of cultured cells from DMBA-induced mammary tumor (DMBA = 9,10-di-methyl-1,2-benzanthracene); derived from identical tumor as HH-16 cl.2/1 (DSM ACC 485); cells were described in the literature to produce invasive adenocarcinomas in S.D. rats upon s.c. injection with remote metastases in lymph nodes and lung |
| Reference(s) ： | 14617, 14925 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | split confluent culture 1:5 to 1:10 twice a week using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-80 hours |
| Harvest ： | cell harvest of ca. 1.0-1.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | rat flat-moded near-tetraploid karyotype with 10% polyploidy - 79-84<4n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |