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| 产品名称： | CAL-54 |
|---|---|
| 商品货号： | TS259478 |
| <strong>Cell line </strong>： | CAL-54 |
| DSMZ no. ： | TS259478 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | kidney carcinoma |
| Origin ： | established from the malignant pleural effusion of a 75-year-old man with kidney carcinoma in 1987 |
| Reference(s) ： | 14566 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | variously shaped, adherent cells growing in monolayers; image; image |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 0.4 µg/ml hydrocortisone + 10 ng/ml EGF |
| Subculture ： | split nearly confluent culture 1:2 to 1:3 about once a week using trypsin/EDTA (it may be difficult to detach the cells from the flask - use double quantity of trypsin/EDTA for ca. 15 min); seed out at ca. 2 x 106 cells/75 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 72 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/75 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 52(52-53)<2n>X, -Y, +2, +3, +7, +7, +8, +12, -13, +15, +16, +17, del(3)(p21), del(5)(p14.2p15.2), dup(7)(q11q22), add(14)(p12) - del(3p), gain of ch 7 and loss of Y typical of renal cell carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |