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| 产品名称： | BFTC-909 |
|---|---|
| 商品货号： | TS259483 |
| <strong>Cell line </strong>： | BFTC-909 |
| DSMZ no. ： | TS259483 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | kidney transitional cell carcinoma |
| Origin ： | established from the sarcomatoid component of a grade III transitional cell carcinoma of the renal pelvis of a 64-year-old man in 1990; patient lived in a blackfoot disease endemic area in Taiwan |
| Reference(s) ： | 15028 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like (spindle) cells growing in monolayers; cells contain considerable amounts of small, dark granules; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:3 to 1:5 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 3-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriplod karyotype with 32% polyploidy - 66-79<3n>XX, -Y, +1, +3, +5, +7, -9, -11, +12, +17, -18, +19, +20, +21, +mar, add(1)(p34)x2, del(2)(p16), +dic(2;9)(q25;p22)x2, add(3)(p24)x2, add(6)(p23)x2, i(7p)x2, i(8q)x2, del(11)(q?22q23), add(12)(q24), add(19)(q13)x2 - 2-4 dmin present in most cells - gain of ch 7 and deletions of 9p typical in TCC - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |