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| 产品名称： | FDCP-1 |
|---|---|
| 商品货号： | TS259484 |
| <strong>Cell line </strong>： | FDCP-1 |
| DSMZ no. ： | TS259484 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, NP, and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | bone marrow |
| Origin ： | established from the long-term bone marrow culture of B6D2F1 mice; cells are IL-3-dependent; their differentiation is blocked; this subclone is responsive to G-CSF (FDCP means "Factor-Dependent Continuous cell lines, Paterson Laboratories" after the Paterson Insitute, Manchester, UK) |
| Reference(s) ： | 14628, 14457 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single or clustered cells growing in suspension |
| Medium ： | 70% Iscoves MDM + 20% horse serum + 10% vol conditioned medium of WEHI-3B cell line (DSM ACC 26), or alternatively 10 ng/ml mIL-3 |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 5 x 105 cells/ml, first 3-4 days after thawing should not be splitted. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypodiploid karyotype - 32<2n> - carries 8 centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |