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| 产品名称： | MEG-01 |
|---|---|
| 商品货号： | TS259486 |
| <strong>Cell line </strong>： | MEG-01 |
| DSMZ no. ： | TS259486 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | chronic myeloid leukemia in megakaryocytic blast crisis |
| Origin ： | established from the bone marrow of a 55-year-old man with chronic myeloid leukemia (CML) in megakaryocytic blast crisis in 1983; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e13-a2 (b2-a2) fusion gene |
| Reference(s) ： | 14479, 14488, 14812, 14813 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing singly or in small clusters in suspension, some cells slightly adherent; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.3 x 106 cells/ml; split saturated culture 1:3 to 1:4 every 3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | maximal density at ca. 1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD15 +, CD19 -, CD33 +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 54(53-56)<2n>XY, +6, +19, +19, +21, +3-4mar, t(1;15)(p13;p13), ?inv(3)(p25q26), i(4q), add(5)(p15), der(9)t(9;22)(q34;q11)x2, add(10)(p14), dup(13)(q13q33-34), add(14)(p11), der(22)t(9;22)(q34;q11) - r/dmin x1 present at 57% - sideline with i(5)(q10)del(5)(q11q13) instead of add(5) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |