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| 产品名称： | KYSE-70 |
|---|---|
| 商品货号： | TS259487 |
| <strong>Cell line </strong>： | KYSE-70 |
| DSMZ no. ： | TS259487 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 77-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria) |
| Reference(s) ： | 15006, 14955, 14954, 14646 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 4-6 days using trypsin/EDTA (cells may need five or more minutes to detach from the flask); prior to using trypsin, cells should be washed twice with PBS) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 60(54-63)<3n>XXX, -Y, -2, -3, -3, -4, -6, +7, -8, -9, -10, -13, -14, -15, -16, -16, -16, -17, -18, -18, -21, -22, +10mar, del(X)(q25), add(1)(p11), add(2)(q33), del(2)(q33), del(3)(q13), add(4)(q35), add(5)(p15), del(5)(q14), add(7)(q36), del(7)(p11), del(8)(p22.2), qdp(9)(p11p24), add(10)(q25), add(11)(p?13), add(19)(p13), i(?22p) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV +, SMRV - |