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| 产品名称： | KYSE-520 |
|---|---|
| 商品货号： | TS259489 |
| <strong>Cell line </strong>： | KYSE-520 |
| DSMZ no. ： | TS259489 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH and later by STR profiling |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the moderately differentiated invasive eosphageal squamous cell carcinoma resected from lower intra-thoracic esophagus of a 58-year-old Japanese woman prior to treatment (tumor had invaded the adventitia); described as carrying p53 mutation and amplification of the oncogene MYC |
| Reference(s) ： | 14954, 14955, 15006, 14646 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayers, occasional giant cells present (ca. 1%); image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:6 to 1:8 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 8% polyploidy - 58-60<3n>XX, -X, +1, +1, -2, -2, -4, -5, -6, +7, -8, -10, -10, -11, -12, -12, -13, -14, -15, -16, -17, -18, +20, +20, -21, -21, -22, -2, +7-8mar, add(X)(p11), add(X)(q11), add(1)(q11), der(1)del(1)(q12)t(1;7)(p36;q21), der(1)del(1)(q12)t(1;5)(p36;q11), del(1)(q21), del(1)(p34), add(2)(q37), del(3)(q13), add(4)(p11), add(4)(p15), add(5)(q11)x1-2, add(6)(q1?), t(6;15)(p10;q10)x1-2, del(7)(q11)x1-2, add(7)(p22), add(9)(p23), der(11)add(11)(p15)t(11;12)(q24;q11), add(11)(p15), add(16)(q2?3), add(17)(q24), add(18)(p11), add(19)(p13)del(22)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |