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| 产品名称： | SCLC-21H |
|---|---|
| 商品货号： | TS259491 |
| <strong>Cell line </strong>： | SCLC-21H |
| DSMZ no. ： | TS259491 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP later by STR finger printinig |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pleural effusion of a 46-year-old Caucasian man with small cell lung carcinoma; sister cell line of cell line SCLC-22H (DSM ACC 373) |
| Reference(s) ： | 14381, 14382 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing in suspension in small or big clusters (sheets), but do not adhere to the plastic surface; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3 - 1.0 x 106 cells/ml; split 1:2 every 2-3 days; cells grow in clumps and are difficult to disperse; viability may decrease after thawing and when dispersed too vigorously; cell counting may be difficult, better after digestion with accutase (sedimented cells, 3-5 ml accutase, 10 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 2 days |
| Harvest ： | cell harvest of ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | identical DNA profiles of SCLC-16HC, SCLC-21H, SCLC-22H and SCLC-24H using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypodiploid karyotype with 12% polyploidy - 42(40-42)<2n>XY, +1, -2, -5, +6, -10, +11, -17, -18, -19, -21, +2mar, del(1)(p22p32), add(5)(p15), del(6)(q14), add(8)(p21), add(11)(q24), del(11)(p11), t(12;17)(q10;q10), del(13)(q12q14), add(15)(q25) - resembles published karyotype (and that of SCLC-16HC) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |