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| 产品名称： | SCLC-22H |
|---|---|
| 商品货号： | TS259494 |
| <strong>Cell line </strong>： | SCLC-22H |
| DSMZ no. ： | TS259494 |
| Species ： | confirmed as human with IEF of AST, LHD, NP |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pericardial effusion of a 46-year-old Caucasian man with small cell lung carcinoma, oat cell type; patient had received chemotherapy; sister cell line of SCLC-21H (DSM ACC 372) |
| Reference(s) ： | 14381, 14382, 14383 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing in suspension in small or very big clusters (sheets) - cells are not adherent; image; image; image |
| Medium ： | 80-90% Dulbeccos MEM + 10-20% h.i. FBS |
| Subculture ： | maintain at ca. 1 x 106 cells/ml; split 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; cells grow in clumps and are difficult to disperse; thus cell counting may be difficult; dispersing the cells may also decrease the viability |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | identical DNA profiles of SCLC-16HC, SCLC-21H, SCLC-22H and SCLC-24H using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypodiploid karyotype with 8% polyploidy - 43(40-44)<2n>XY, -8, -10, +11, +12, -16, -17, del(4)(q32), add(5)(p15), del(11)(p11), t(12;17)(q10;q10), add(12)(q23), del(13)(q12q14), add(21)(p11), add(22)(q13) - sideline with del(3)(p14) - closely resembles karyotype of sister cell line SCLC-21H |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |