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| 产品名称： | SR-786 |
|---|---|
| 商品货号： | TS259496 |
| <strong>Cell line </strong>： | SR-786 |
| DSMZ no. ： | TS259496 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP and later by STR fingerprinting |
| Cell type ： | anaplastic large T cell lymphoma |
| Origin ： | established from the pleural effusion an 11-year-old boy with CD30+ (Ki-1) large T cell lymphoma in 1983 (also described as "SR"); described in the literature to express the NPM1-ALK (NPM-ALK) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14375, 14464, 14990, 14991, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | ovoid-to-round, sometimes irregular, occasionally with apical processes, growing in suspension, singly or in small clumps; image; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:2.5 every 3-4 days; to maximize viability, it may be necessary to replenish medium daily; cultures show a temporary loss of viability from one to five days after recovery of frozen ampoules |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximum density of ca. 0.9-1.3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 -, CD4 +, CD7 -, CD8 -, CD10 -, CD13 -, CD19 -, CD30 +, CD71 +, HLA-DR +, TCRalpha/beta -, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid, flat-moded karyotype - 70-84<3n>XX, +1, +2, -4, +5, +6, +7, +8, -13, -13, +14, -18, +19, +22, +6-9mar, add(1)(q11), del(1)(p11)/der(?)t(1;?)(q11;?), der(2)t(2;5)(p23;q35)inv(2)(p23q14)x2, del(4)(q22), der(5)t(2;5)(p23;q35)x2, del(7)(q21), der(9)t(1;9)(q11;p24)x2, der(12)t(12;13)(q24.32;q11)x1-2, del(13)(q13q31), add(14)(p11)/der(?)t(14;?)(q11;?)x2, del(21)(q22) - carries masked t(2;5) associated with ALCL |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |