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| 产品名称： | KYSE-150 |
|---|---|
| 商品货号： | TS259500 |
| <strong>Cell line </strong>： | KYSE-150 |
| DSMZ no. ： | TS259500 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MDH, later by STR-typing |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the poorly differentiated esophageal squamous cell carcinoma resected from upper (cervical) esophagus of a 49-year-old Japanese woman after receiving radiotherapy (tumor was invading contiguous structures); described as carrying amplified oncogenes, c-erb-B (8x) and cyclin D1 (4x) and producing tumors in nude mice |
| Reference(s) ： | 14646, 14954, 14955, 15006 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as adherent monolayer; image; image |
| Medium ： | 49% RPMI 1640 + 49% Hams F12 + 2% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/175 cm2; split confluent culture up to 1:20 every 3-4 days using trypsin/EDTA; cells grow very fast, a FBS amount around 2-5% is sufficient |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | below 25 hours (even less when serum amount is increased) |
| Harvest ： | cell harvest of ca. 15 x 106 cells in a 175cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 12% polyploidy - 70(68-75)<3n>XderX, -X, +1, -3, -4, -8, -9, +11, -14, -15, -16, -18, -21, +12mar (4xbisat), add(X)(q27), inv(1)(p34q32), del(1)(p32), del(1)(q11), del(7)(q32), del(8)(p21), i(9q), add(9)(q34), add(11)(p11), der(11)add(11)(p1?)add(11)(q24), del(1)(p11), del(12)(q21), del(15)(q23q25), add(19)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |