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| 产品名称： | KYSE-510 |
|---|---|
| 商品货号： | TS259503 |
| <strong>Cell line </strong>： | KYSE-510 |
| DSMZ no. ： | TS259503 |
| Species ： | originally confirmed as human with IEF of AST, LDH |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the well-differentiated eosphageal squamous cell carcinoma with moderate invasion of the adventitia resected from cervical esophagus of a 67-year-old Japanese woman after cisplatin and radiotherapy; described as carrying p53 mutation and amplification of MYC, HST1, CYCLIN D1, and to induce well-differentiated tumors in nude mice |
| Reference(s) ： | 14646, 15006, 14954, 14955 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as monolayer with multilayer foci; image; image |
| Medium ： | 90% RPMI 1640 (or mixture of RPMI 1640 + Hams F12 at 1:1) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-3.0 x 106 cells/175 cm2; split confluent culture 1:5 to 1:20 every 4-8 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 15-25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 73(66-73)<3n>XXX, -X, +1, +1, -2, -2, +3, -4, +5, +6, +7, -14, -14, +19, -21, -21, +22, +9mar, add(X)(p22.2), add(1)(q21), ider(1)(q10)t(1;8)(q21;q11), add(2)(q11), del(3)(q21), del(3)(p1?), add(5)(p14), i(8p), der(8)add(8)(p11)add(8)(q24), i(9p), add(9)(p11), add(11)(p15), add(11)(q13)x2, add(14)(p11), add(15)(p11)x2, der(18)add(18)(p11)dup(18)(q11q23)add(18)(q23), der(18)add(18)(p11)add(18)(q2?3), der(18)add(18)(p11)add(8)(q2?1), del(19)(p13)x2, der(19)t(10;19)(q11;q13), add(21)(p11), del(22)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |