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| 产品名称： | TCC-SUP |
|---|---|
| 商品货号： | TS259504 |
| <strong>Cell line </strong>： | TCC-SUP |
| DSMZ no. ： | TS259504 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from the tumor specimen resected from the urinary bladder transitional cell carcinoma (undifferentiated, grade IV) of a 67-year-old woman in 1974 (without therapy) |
| Reference(s) ： | 14803, 14579 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells with smooth borders growing as monolayer |
| Medium ： | 80-85% Dulbeccos MEM (4.5 g/L glucose) + 15-20% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 6 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 67(58-67)<3n>XX, -X, +1, -2, -4, +5, -9, -12, -18, -21, +1-2mar, der(1;21)(q10;q10), del(1)(q13), i(5p)x3, del(6)(q14), add(6)(p23)/der(6)t(3;6)(p22;p22), add(6)(p22/23), del(10)(p11), add(20)(q12), add(21)(p11/12) - i(5p) and rearrangements of chs 1 and 3p recurrent in bladder TCC - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |