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| 产品名称： | KU-812 |
|---|---|
| 商品货号： | TS259507 |
| <strong>Cell line </strong>： | KU-812 |
| DSMZ no. ： | TS259507 |
| Species ： | confirmed originally as human with IEF of AST, LDH, MDH |
| Cell type ： | chronic myeloid leukemia in myeloid blast crisis |
| Origin ： | established from the peripheral blood of a 38-year-old man with chronic myeloid leukemia (CML) in myeloid blast crisis; described in the literature to express basophilic features and to carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14479, 14488, 14663, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension ; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | cells grow very slowly; seed out at ca. 0.5 x 106 cells/ml; after thawing, cells may have a lag phase of 1-2 weeks before reassuming vigorous growth; then, maintain at 0.4-0.8 x 106 cells/ml; split 1:2 every 3-4 days; viability may not exceed 80% |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | maximum density of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD33 +, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 8% polyploidy - 61(58-62)<3n>XYY, -2, -3, -5, +6, -7, +8, -10, -12, -16, -17, -18, +19, -20, t(9;22)(q34;q11)x2, i(11q), i(17q) - Ph (two copies) together with i(17q) and additional chs 8 and 19 - matches published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |