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| 产品名称： | KYSE-270 |
|---|---|
| 商品货号： | TS259510 |
| <strong>Cell line </strong>： | KYSE-270 |
| DSMZ no. ： | TS259510 |
| Species ： | confirmed originally as human with IEF of LDH, MDH, NP and later by STR typing |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 79-year-old Japanese man prior to treatment (depth of invasion was not beyond the muscularis propria); described in the literature to carry p53 mutation |
| Reference(s) ： | 14954, 14955, 15006, 14646 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing in monolayers; image; image; image |
| Medium ： | 49% RPMI 1640 + 49% Hams F12 + 2% h.i. FBS |
| Subculture ： | seed out at ca. 0.5-2 x 106 cells/80 cm2; split confluent culture 1:5 every second day using trypsin/EDTA (incubation for 10-15 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 16% polyploidy - 66(64-67)<3n>XX/XXX, +1, -2, +3, -5, -6, +7, +8, -9, -10, -15, -15, -17, -18, -19, -20, -22, +5mar (1bisat), ?del(X)(q25), add(1)(p1?), add(1)(p2?), i(1p), der(1)t(1;?)(?;2)(q13;?)(?;q11), add(2)(q37), del(2)(q23), add(3)(p2?6)x2, i(5p), add(5)(p15), del(7)(p11), add(8)(q24), add(16)(q24), add(18)(q23), add(19)(p13), add(20)(q12), add(21)(p11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |