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| 产品名称： | YAPC |
|---|---|
| 商品货号： | TS259513 |
| <strong>Cell line </strong>： | YAPC |
| DSMZ no. ： | TS259513 |
| Species ： | confirmed originally as human with IEF of AST, NP and recently by STR typing |
| Cell type ： | pancreas carcinoma |
| Origin ： | established from ascites obtained during exploratory laparatomy of a 43-year-old Japanese man with pancreas carcinoma (terminal, no therapy) in December 1993; cells were described as being dependent on autocrinely secreted IL-1alpha |
| Reference(s) ： | 15070 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cobblestone-like adherent cells growing in monolayers; image |
| Medium ： | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/ 175 cm2; split culture up to 1:10 every 4 to 6 days prior to confluence (as the monolayer detaches upon reaching confluency) using trypsin/EDTA (incubate with trypsin/EDTA for 10-20 min); optimal density is at about 5 x 106 cells/175 cm2; it is recommended to add 10% of the spent medium to any fresh culture; after thawing cells may have a lag time of 1-2 days before reassuming growth |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | cell harvest of ca. 2.0 x 107 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32), add(9)(p1?), der(9;15)(q10;q10), der(10)t(10;11)(p11;q13)x2, del(12)(p12), add(13)(p11)x2, add(13)(p11), inv(16)(p13q11.2), del(18)(q21), add(20)(p11)/der(20)t(1;20)(p34;p11) - loss of 1p, 3p, 9p, 18q associated with pancreas carcinoma - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |