微信小李
微信小章
产品简介
购买须知
| 产品名称： | EPLC-272H |
|---|---|
| 商品货号： | TS259514 |
| <strong>Cell line </strong>： | EPLC-272H |
| DSMZ no. ： | TS259514 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH and recently by STR-fingerprinting |
| Cell type ： | epidermoid lung carcinoma |
| Origin ： | established from a surgical lung specimen of a 57-year-old Caucasian man who underwent surgery for undifferentiated squamous cell carcinoma of the lung (without having received prior radiation or chemotherapy) in 1986 |
| Reference(s) ： | 14384 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells (without sharp contours) growing adherently as monolayers |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA (incubate for >10 min) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 5-7 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 66(59-70)<3n>XX, -Y, -4, +7, +8, -18, -19, -20, -21, +6mar, add(1)(p32), del(2)(q33), der(2;11)(q10;q10), add(3)(p25), i(5p), i(5q), add(6)(q11), add(7)(q33)x2-3, add(7)(p15), del(8)(p12-21), del(10)(p14)x1-2, add(11)(q24)x2, add(12)(p11-12), add(13)(q33), add(14)(p12), i(15q), der(17)t(3;17)(p11;p12), add(19)(q13), add(21)(p12), dmx2 - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |