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| 产品名称： | KYSE-410 |
|---|---|
| 商品货号： | TS259515 |
| <strong>Cell line </strong>： | KYSE-410 |
| DSMZ no. ： | TS259515 |
| Species ： | originally confirmed as human with IEF of AST, LDH, MDH and recently by DNA fingerprinting |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the poorly differentiated invasive esophageal squamous cell carcinoma resected from the cervical esophagus of a 51-year-old Japanese man prior to treatment (tumor invasion into the adventitia was obvious); described in the literature to overexpress hst-1 (= heparin-binding growth factor) and cyclin D1 |
| Reference(s) ： | 14646, 14954, 14955, 15006 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelioid adherent cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 4% polyploidy - 79-91<4n>Xder(X), -10, -18, -19, -21, -22, -22, +6mar, add(X)(qter), add(1)(p11)/del(1)(p13), add(3)(q21)x1-2, add(5)(p13), der(6)t(4;6)(q12;q14)x1-2, dup(7)(p12p14), der(7)del(7)(p12p21)t(7;9)(q33;q10), add(8)(p12)x1-2, add(9)(q34), add(10)(p14), add(11)(p11), add(12)(q24)x1-2, add(12)(p11-12)x2, del(12)(q14), i(13q)x2, i(14q)x1-2, add(14)(p11), add(19)(p13), add(?20)(q13), add(21)(p11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |