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| 产品名称： | KYSE-180 |
|---|---|
| 商品货号： | TS259516 |
| <strong>Cell line </strong>： | KYSE-180 |
| DSMZ no. ： | TS259516 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the well differentiated esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 53-year-old Japanese man prior to treatment (tumor was invading contiguous structures) |
| Reference(s) ： | 14955, 15006, 14954, 14646 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as adherent monolayer; image; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells/80 cm2 in 10-15 ml; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 11% polyploidy - 55(50-57)<2n>XX, +1, +1, +3, +7, +7, +8, -14, +16, -17, -17, -18, -18, +5-8mar, del(X)(q23), add(1)(q32), der(1;6)(q10;p10), der(1;13)(q10;q10), add(3)(p26), add(5)(q25), add(6)(p11), del(6)(q23), add(7)(p11), del(7)(p11), add(7)(q35), i(7q), del(8)(p22), add(10)(p11), add(11)(q11), del(11)(q11q14), add(13)(p11), add(13)(q33), add(14)(p11), add(15)(p11), add(21)(p11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |