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| 产品名称： | EOL-1 |
|---|---|
| 商品货号： | TS259520 |
| <strong>Cell line </strong>： | EOL-1 |
| DSMZ no. ： | TS259520 |
| Species ： | originally confirmed as human with IEF of LDH, MDH, NP later by STR fingerprinting |
| Cell type ： | acute myeloid (eosinophilic) leukemia |
| Origin ： | established in 1984 at diagnosis from the peripheral blood of a 33-year-old man with acute myeloid (eosinophilic) leukemia (AML) following hypereosinophilic syndrome; we found that EOL-1 carries a KMT2A (MLL) partial tandem duplication; described in the literature to carry the fusion FIP1L1-PDGFRA. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14439, 14751, 14868, 14912, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | (relatively small) round cells growing in suspension, singly or in small clusters; image; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; start culture with 20% FBS; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | maximum density of ca. 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 7.5% polyploidy - 50(48-51)<2n>,XY, +4, +6, +8, +19, del(4)(q12)x2, del(9)(q22) - del(4) effects cryptic FIP1L1-PDGFRA fusion - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |