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| 产品名称： | KYSE-450 |
|---|---|
| 商品货号： | TS259521 |
| <strong>Cell line </strong>： | KYSE-450 |
| DSMZ no. ： | TS259521 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting |
| Cell type ： | esophageal squamous cell carcinoma |
| Origin ： | established from the well differentiated invasive eosphageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 59-year-old Japanese man prior to treatment (depth of invasion was not beyond the submucosa); described as carrying p53 mutation and amplification of the oncogene MYC |
| Reference(s) ： | 14646, 15006, 14954, 14955 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayers; image; image; image |
| Medium ： | 45% RPMI 1640 + 45% Hams F12 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/25 cm2; split confluent culture 1:8 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 6.7% polyploidy - 57(49-57)<2n>, -Y, +1, +3, +3, +7, +12, +17, -18, +7mar, der(1)t(1;6)(p22;q11), add(1)(p32/34), del(2)(p23), del(3)(p22), del(4)(q21), add(5)(q35), del(6)(q25), add(7)(p22), add(8)(p23), add(9)(q34), del(11)(q14q21), add(12)(q14), del(12)(p11p12), der(14)t(8;14)(q21;p11), del(17)(p11), der(19)t(1;19)(q11;q12) - sideline with add(11)(q?23) - extensive subclonal rearrangements of ch 7 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |