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| 产品名称： | LCLC-103H |
|---|---|
| 商品货号： | TS259526 |
| <strong>Cell line </strong>： | LCLC-103H |
| DSMZ no. ： | TS259526 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | large cell lung carcinoma |
| Origin ： | established from the pleural effusion of a 61-year-old Caucasian man with large cell lung carcinoma with giant cells who had received chemo- and radiotherapy; described in the literature to be PAS negative, to exhibit remarkable stroma formation and to overexpress the proto-oncogene MYC |
| Reference(s) ： | 14382, 14384 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | pleomorphic, gigantic, adherent cells growing as monolayer; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:2 to 1:3 twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 9-13 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human, highly rearranged flat-moded hexaploid/heptaploid karyotype with 4% polyploidy - 130-160<7n>XXY, -X, -Y, -Y, +1, +1, -2, -2, -3, -6, -6, -8, -8, -9, -9, -9, -10, -13, -13, -13, -14, -14, +15, +16, +16, -17, -18, -19, -19, -20, -20, -20, -20, +18-25mar, del(1)(p22.2), add(1)(q11-21), i(1p)x2, add(3)(p23-24)x2, del(5)(q11q15), i(5q)x2, add(5)(p?), add(6)(q11)x2-3, add(7)(q34)x2, del(7)(q22)x2, i(8q), del(9)(p21)x2-3, del(10)(q23-24)x2, der(10;13)(q10;q10)x2, add(11)(q24-25)x2, del(11)(q23)x2, add(13)(p11), add(16)(q24) - variable number of dmin present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |