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| 产品名称： | CMK |
|---|---|
| 商品货号： | TS259530 |
| <strong>Cell line </strong>： | CMK |
| DSMZ no. ： | TS259530 |
| Species ： | originally confirmed as human with IEF of AST, NP and later by STR fingerprinting |
| Cell type ： | acute megakaryocytic leukemia |
| Origin ： | established from the peripheral blood of a 10-month-old boy with Downs syndrome and acute megakaryocytic leukemia (AML M7) at relapse in 1985. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14685, 14921, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, multiformed, large cells growing in suspension, sometimes loosely adherent (<3%); 2-3% giant, multinucleated cells; image; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; viability may be consistently between 60% and 80% and culture may always contain a certain amount of cell debris; only exchange spent medium partially during the first week; cultivation in 12- or 24-well plates could be of advantage |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | maximal density at ca. 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 +, CD41 +, CD71 +, CD235a +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 8% polyploidy - 85-90<4n>XY, -X, -Y, -2, -3, +5, -6, -6, -8, +11, -15, -15, +16, -17, -19, +21, +22, +7-11mar, add(1)(q31), add(1)(p36), add(3)(q11), del(3)(p14)x2-3, add(5)(q11), add(5)(q13), dup(8)(q11q21), add(8)(q13-21), del(8)(q11), del(9)(p21)x2, add(9)(q11)x2, del(10)(q22q24), der(11;17)(q10;q10), der(11)dup(11)(p13p15)t(5;11)(q11;p15)x1-2, del(11)(q23), add(12)(p13)x2, add(17)(p1?), add(18)(q23)x2-3, add(19)(p13), der(20)t(1;20)(q2?5;q1?2)x2, add(22)(q13) - disomic rearrangement of 20q1 close to common deleted segment, together with additional rearrangements of ch 20 present in markers - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |