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| 产品名称： | RMB-1 |
|---|---|
| 商品货号： | TS259531 |
| <strong>Cell line </strong>： | RMB-1 |
| DSMZ no. ： | TS259531 |
| Species ： | originally confirmed as murine with IEF of AST, LDH, MDH and NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | myeloid leukemia |
| Origin ： | tumor induced in nude mouse injected with blast cells from ascites fluid of newborn Balb/c mouse injected intraperitoneally with Rauscher murine leukemia virus (supernate of producer cell line JLS-V9); descibed to be heterotransplantable, to form colonies in soft agar, to display myeloid cytochemical (myeloperoxidase) and functional (phagocytosis) features, and to differentiate terminally in response to DMSO |
| Reference(s) ： | 14450 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spheroidal cells growing singly in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | maintain at 1-2 x 106 cells/ml; split saturated culture 1:4 to 1:10 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-35 hours |
| Harvest ： | maximal density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hyperdiploid karyotype - 41(38-41)<2n>XY, structurally and numerically rearranged |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |