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| 产品名称： | MSTO-211H |
|---|---|
| 商品货号： | TS259534 |
| <strong>Cell line </strong>： | MSTO-211H |
| DSMZ no. ： | TS259534 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | mesothelioma |
| Origin ： | established from the pleural effusion of a 62-year-old Caucasian man with mesothelioma (malignant biphasic) who did not have any prior therapy |
| Reference(s) ： | 14382 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblast-like cells growing as monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 every 3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype - 79(77-80)<3n>XYY/XXYY, +2, +4, +7, +7, +7, -10, +12, +13, +14, -15, -15, +16, +16, +16, -17, +18, -22, +1-2mar, 1-20dmin - add(1)(p35-36), add(1)(p34), der(4)t(4;17)(p14;q21)x2, del(6)(q25)x2, del(7)(q11), del(7)(q21.2)x2, inv(9)(p24q34), del(10)(q24), add(14)(p11), add(15)(p11), i?dic(16)(q10)x2 - sideline with der(6;9)(p10;q10) - high 7p copy number associated with poor prognosis mesothelioma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |