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| 产品名称： | SUP-B15 |
|---|---|
| 商品货号： | TS259537 |
| <strong>Cell line </strong>： | SUP-B15 |
| DSMZ no. ： | TS259537 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP |
| Cell type ： | B cell precursor leukemia |
| Origin ： | established from the bone marrow of a 9-year-old boy with acute lymphoblastic leukemia (B cell precursor ALL) in second relapse in 1984; described in the literature to carry the ALL-variant (m-bcr) of the BCR-ABL1 fusion gene (e1-a2) |
| Reference(s) ： | 14488, 14800 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small round cells growing singly in suspension; image; image |
| Medium ： | 80% McCoys 5A + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; it is advisable to culture the cells initially in 24-well-plates, cell growth may be slow during the first week after thawing; maintain at ca. 0.5-2.0 x106 cells/ml; split 1:2 to 1:3 about once or twice a week |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 1.5 x 106 cells/ml; maximum density of ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD19 +, CD34 +, CD37 -, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, smIgM -, cyIgM +, sm/cykappa -, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human pseudodiploid karyotype - 46<2n>XY, der(1)t(1;1)(p11;q31), add(3)(q2?7), der(4)t(1;4)(p11;q35), t(9;22)(q34;q11), add(10)(q25), ?del(14)(q23q31), der(16)t(9;16)(q11;p13) - resembles published karyotype |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 (e1-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |