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| 产品名称： | BE-13 |
|---|---|
| 商品货号： | TS259539 |
| <strong>Cell line </strong>： | BE-13 |
| DSMZ no. ： | TS259539 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from bone marrow cells of an 11-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) in relapse; cell lines BE-13 and PEER (DSM ACC 6) show identical DNA fingerprints and hence share common origin, presumably due to cross-contamination; however, their diploid and tetraploid karyotypes indicative of earlier and later passage numbers, respectively, suggest that BE-13 is derived from PEER, rather than vice-versa; carries NUP214-ABL1 fusion gene |
| Reference(s) ： | 14574, 15136 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at about 1 x 106 cells/ml in a 24- or 6-well plate; maintain at 0.5-2.0 x 106 cells/ml; split 1:2 to 1:3 every 2-4 days; (cells grow clearly better in 24- or 6-well plates than in flasks) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-60 hours |
| Harvest ： | maximal density at ca. 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile, but identical to DNA profile of PEER |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype - 81-89<4n>XXXX, +1, +4, -9, -9, -10, -16, -21, del(1)(q13.2), add(1)(p11-21), del(4)(q11q31.2)/i(4p)x2, i(4q)del(4q31.2)/i(4q)add(4)(q31)x2, del(5)(q14q21)x2, del(6)(q22)x2, del(9)(p22)x2, der(17)t(17;?21)(p11;q11)x2 - 5q- and apparent 9p22-pter nullisomy |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |