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| 产品名称： | HT-1376 |
|---|---|
| 商品货号： | TS259543 |
| <strong>Cell line </strong>： | HT-1376 |
| DSMZ no. ： | TS259543 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | urinary bladder carcinoma |
| Origin ： | established from a transurethral resection of invasive, moderately pleomorphic (grade 3) bladder transitional cell carcinoma of a 58-year-old Caucasian woman who had not received chemo- or radiotherapy |
| Reference(s) ： | 14579, 14882 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing in monolayers |
| Medium ： | 85% Dulbeccos MEM (4.5 g/L glucose) + 15% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent cultures 1:5 once a week using trypsin/EDTA; seed out at ca. 1.5 x 106 cells/80 cm2; maximal density at ca. 60 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 60 hours |
| Harvest ： | cell harvest of ca. 40 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded pentaploid karyotype with 8% polyploidy - 111(102-116)<5n>XX/XXX, +3, +3, +5, +5, -6, +7, +8, +8, -12, -13, -13, -14, -14, -15, -15, -15, -15, -16, -17,-17, -19, -19, +20, -22, -22, -22, +6-8mar - del(2)(q25), add(3)(q27)x2, del(3)(p11p14), i(5q), add(5)(p15), del(5)(p13), add(5)(q1?3;hsr), del(6)(q25), add(7)(p22), del(7)(q11), add(8)(p23)x2, i(9q)x2, del(9)(p21), del(10)(q24), add(11)(p15), add(12)(p13)x1-2, del(12)(p11), der(13)t(13;?)(p11;?)t(?;1)(?;q11), add(17)(p1?), add(18)(p11) - sideline with i(5p) - 3p, 5p/q10, +7, 8p, 9p, 10q, 11p, 17p rearrangements associated with bladder TCC - matches published karyotypes |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |