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| 产品名称： | BD-215 |
|---|---|
| 商品货号： | TS259544 |
| <strong>Cell line </strong>： | BD-215 |
| DSMZ no. ： | TS259544 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | B lymphoblastoid cells (from patient with Fanconi anemia) |
| Origin ： | B lymphoblastoid cell line (B-LCL) established from the peripheral blood of a Scottish man with Fanconi anemia (complementation group C); homozygote with unrelated non-consanguineous parents; homozygous for nonsense mutation in exon 6 of the FANCC (FACC) coding sequences |
| Reference(s) ： | 14559 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid-like cells growing as single cells or in clumps in suspension |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:5 every 2-4 days; maintain at 0.4-1.0 x 106 cells/ml; seed out at ca. 0.8 x 106 cells/ml; after recovery from the frozen stock, first culture with 20% FBS |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | saturation density at ca. 2.0-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 +, HLA-DR +, sm/cyIgM -, smIgG -, cyIgG +, sm/cykappa -, sm/cylambda - |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human diploid karyotype with 12% polyploidy - 46, XY, 1qh+ - no consistent abnormality detected |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |