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| 产品名称： | LOU-NH91 |
|---|---|
| 商品货号： | TS259546 |
| <strong>Cell line </strong>： | LOU-NH91 |
| DSMZ no. ： | TS259546 |
| Species ： | confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | lung squamous cell carcinoma |
| Origin ： | established from the lower right lung lobe removed two months after diagnosis of highly differentiated squamous cell lung carcinoma with metastasis to one lymph node (no visceral involvement) in a 64-year-old woman (without any prior therapy) |
| Reference(s) ： | 14494 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent ovoid-to-epitheloid cells growing in monolayers; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:4 every 5-10 days using trypsin/EDTA (cells require longer than usual to detach after trypsinization, ca. 5 min); seed out at ca. 0.5-1.0 x 106 cells/80 cm2; doubling time may initially be longer after recovery from N2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | cell harvest of ca. 1.0-1.5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with declining hyperhaploid sideline (2%) and 9% polyploidy - 50-53<2n>XX/XXXX, +5, +5, +7, +7, -13, +17, -18, +22, +22, +1-2mar, del(4)(q27), ?add(22)(q13)x2 (major clone) - 54<2n>XXXX, +5, +5, +7, +7, +22, +22 (minor clone) - 27 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |