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| 产品名称： | LOUCY |
|---|---|
| 商品货号： | TS259547 |
| <strong>Cell line </strong>： | LOUCY |
| DSMZ no. ： | TS259547 |
| Species ： | originally confirmed as human with IEF of AST, NP and later by STR fingerprinting |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 38-year-old woman with T cell acute lymphoblastic leukemia, T-ALL (FAB L2) (chemotherapy-resistant, remission was never achieved); cell line expresses the SET-NUP214 fusion transcript |
| Reference(s) ： | 14379, 15868, 16550, 16557 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, rather small cells growing singly in suspension ; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximal density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 16% polyploidy - 45<2n>X, -X, del(1)(p3?2p3?4), del(5)(q14-15q34-35), t(16;20)(p1?1;q1?3) - sideline with del(6)(q23) - 1p32, 1p34 and 6q- recurrent in T-ALL - resembles published karyotype |
| Molec. Genetics ： | expression of MEF2C confirmed by RT-PCR and Western blot |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |