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| 产品名称： | FDCP-Mix cl.A4 |
|---|---|
| 商品货号： | TS259551 |
| <strong>Cell line </strong>： | FDCP-Mix cl.A4 |
| DSMZ no. ： | TS259551 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | bone marrow |
| Origin ： | established from the long-term bone marrow cultures of BDF1 mice; cells are mIL-3-dependent (FDCP means "Factor-Dependent Continuous cell lines, Paterson Laboratories" after the Paterson Institute, Manchester, UK); it is described that cells can be maintained in a primitive state or induced to differentiate |
| Reference(s) ： | 14977, 14978 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells in suspension, sometimes in small clusters; image; image |
| Medium ： | 80% Iscoves MDM + 20% h.i. FBS + 10 ng/ml murine IL-3 (or 10-20% conditioned medium of cell line WEHI-3B, DSM ACC 26) |
| Subculture ： | split saturated culture 1:2 every 3-4 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells are initially very difficult to culture and viability may be initially rather low (20-30%): hence it is advisable to culture the cells first in 24-well-plates and to exchange the medium, adding fresh mIL-3, already about 5-8 hours after thawing and then daily. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | maximum density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 10% polyploidy - 40(38-40)<2n> - fusion markers absent |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |