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| 产品名称： | MT-3 |
|---|---|
| 商品货号： | TS259552 |
| <strong>Cell line </strong>： | MT-3 |
| DSMZ no. ： | TS259552 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | colon carcinoma (derivative of LS-174T) |
| Origin ： | allegedly derived "from surgical material of a human breast carcinoma; the tumor was passaged in nude mice prior to adaptation to continuous growth in culture vessels"; but DNA fingerprinting showed cross-contamination with LS-174T; LS-174T was established in 1974 from the moderately well-differentiated primary colonic adenocarcinoma (Duke´s type B) of a 58-year-old woman |
| Reference(s) ： | 14802, 15208 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells, after splitting cells start to grow in colonies (not necessarily as monolayer) |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate |
| Subculture ： | split confluent culture 1:10 every 4-5 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | cell harvest of ca. 0.4 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 47(45-48)<2n>X, -X, +7, +22, i(13q), del(22)(q11) - trisomy 7 recurrent in breast cancer - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |