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| 产品名称： | TANOUE |
|---|---|
| 商品货号： | TS259557 |
| <strong>Cell line </strong>： | TANOUE |
| DSMZ no. ： | TS259557 |
| Species ： | confirmed as human with IEF of AST, LHD, NP |
| Cell type ： | B cell leukemia |
| Origin ： | established from the peripheral blood of an 11-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990 |
| Reference(s) ： | 14503 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, small, round cells growing in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 1-2 x 106 cells/ml; split ratio of 1:3 to 1:4 once a week; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | maximal density at ca. 3-5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 (+), CD38 +, cyCD79a +, CD138 +, HLA-DR (+); image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 47/48<2n>X, -Y/XYqh+, +7, +14, dup(1)(q21.1/21.2q23.1/23.2), t(2;4)(q2?2;q2?5), del(6)(q27), t(8;14)(q24;q32), del(13)(q34), add(22)(q13) - extensive subclonal variation - carries t(8;14), +7, and dup(1q) typical of B-ALL/Burkitt lymphoma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |