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| 产品名称： | GMS-10 |
|---|---|
| 商品货号： | TS259559 |
| <strong>Cell line </strong>： | GMS-10 |
| DSMZ no. ： | TS259559 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | glioblastoma |
| Origin ： | established from the malignant brain tumor of a 49-year-old man with glioblastoma in 1995 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like cells growing as monolayers; image; image; image |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/80 cm2 ; split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50-70 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 11% polyploidy - 69-76<3n>XXYY, +Y, +2, +3, +9, -10, -12, +15, +15, -18, +20, +20, -22, +2-4mar, +1-4dmin, del(2)(p11), add(2)(q31;hsr), der(4)t(3;4)(p21;p15.3), add(4)(p1?), del(6)(q23), add(7)(q11), add(9)(p23), add(13)(p11), add(15)(p11)x3, add(19)(q13), add(21)(p11)x3 - same hsr present in mar and add(2) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |