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| 产品名称： | RED-1 |
|---|---|
| 商品货号： | TS259561 |
| <strong>Cell line </strong>： | RED-1 |
| DSMZ no. ： | TS259561 |
| Species ： | originally confirmed as mouse with IEF of AST and NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | erythroid leukemia |
| Origin ： | established from a Rauscher murine leukemia virus-induced erythroid leukemia of a female DBA/2 mouse; cells were described in the literature to be responsive to erythropoietin (EPO) and to form benzidine-positive colonies upon stimulation with EPO or DMSO |
| Reference(s) ： | 14589 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round, single cells growing in suspension |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | maintain at 0.5 x 106 cells/ml; split saturated culture at 1:4 to 1:5 every second day; seed out at ca. 0.5-1.0 x 106 cells/ml; viability immediately after thawing may be rather low (<30%), but cells recover quickly and viability will raise over the next few passages; maximal density at ca. 2-3 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 1-2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypodiploid karyotype with 10% polyploidy - 39(38-40)<2n>, t(X;1)(q10;q10) - two centric-fusion markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |