微信小李
微信小章
产品简介
购买须知
| 产品名称： | GOS-3 |
|---|---|
| 商品货号： | TS259563 |
| <strong>Cell line </strong>： | GOS-3 |
| DSMZ no. ： | TS259563 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | glioblastoma (derivative of U-343-MG) |
| Origin ： | originally described as a glioma cell line established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG; described in the literature to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation |
| Reference(s) ： | 14590, 14591, 19087 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblastoid, adherent cells growing as monolayer; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 4 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:3 every third day using trypsin/EDTA; maintain at 7-10 x 106 cells/175 cm2; seed out at ca. 1 x 106 cells/25 cm2 in 10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 10-15 x 106 cells/175 cm2; maximal density of 17 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | fluorescent nonaplex PCR of short tandem repeat markers revealed an identical DNA profile as U-343-MG |
| Cytogenetics ： | human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 typical in low-grade astrocytoma-oligodendroglioma - gains of sex chromosomes (particularly Y) are very rare in tumor cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |