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| 产品名称： | U-2197 |
|---|---|
| 商品货号： | TS259564 |
| <strong>Cell line </strong>： | U-2197 |
| DSMZ no. ： | TS259564 |
| Species ： | confirmed originally as human with IEF of AST, NP and later by STR fingerprinting |
| Cell type ： | malignant fibrous histiocytoma |
| Origin ： | established from the subcutaneous malignant fibrous histiocytoma of a 79-year-old Caucasian man in 1984 (third recurrence after repeated surgery, radially invading the thumb extensor); described in the literature to express high levels of PDGF-A which is not secreted |
| Reference(s) ： | 14348, 14553 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid large polygonal cells with extensive lamellae; image; image; image |
| Medium ： | 80% MEM (with Earles salts) + 20% h.i. FBS + 0.13% Na-bicarbonate |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 once a week using trypsin/EDTA; maintain at 6 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 100-120 hours |
| Harvest ： | cell harvest of ca. 8 x 106 cells/175 cm2; maximal density of 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype with hypotriploid sideline and 20% polyploidy - 100(92-101)<4n>XXXX, -2, -4, +6, +6, +7, -8, -9, -9, +14, +14, -15, -15, -16, -16, +19, -20, -20, -21, -21, +13mar, i(Xq)x2, add(X)(p11)x2, del(1)(q11)x2, del(4)(p11), add(6)(q15)x2, i(6p)x2-4, add(7)(q35)x2, add(7)(p14)x2, del(7)(p11), add(12)(q24)x2, add(13)(p11)x2, add(13)(p11)x2, add(19)(q13), del(19)(q13)x2 - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |