微信小李
微信小章
产品简介
购买须知
| 产品名称： | BHY |
|---|---|
| 商品货号： | TS259567 |
| <strong>Cell line </strong>： | BHY |
| DSMZ no. ： | TS259567 |
| Species ： | originally confirmed as human with IEF of AST, NP and recently by STR fingerprinting |
| Cell type ： | oral squamous cell carcinoma |
| Origin ： | established from the tumor of a 52-year-old Japanese man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice |
| Reference(s) ： | 14652 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L gluocse) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 in 15 ml medium; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | cell harvest of ca. 7 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del(5)(q13), add(5)(p15)x1-2, der(8;10)(q10;q10), add(9)(q34), add(9)(p11), del(10)(p13), del(10)(q23), der(11)t(1;11)(q11;q25), add(12)(p11), add(12)(p11), add(12)(q13-21), add(13)(p11), add(16)(q24), del(16)(p13), add(17)(p12), add(18)(p11), add(19)(q13) - 3-10 dmin counted in some cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV+, SMRV - |