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| 产品名称： | RT-4 |
|---|---|
| 商品货号： | TS259570 |
| <strong>Cell line </strong>： | RT-4 |
| DSMZ no. ： | TS259570 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | urinary bladder transitional cell carcinoma |
| Origin ： | established from a recurrent well-differentiated transitional papillary tumor of the urinary bladder (clinical stage T2, histological grade G1, treated with gold grains 2 years earlier and diathermy after recurrence 10 months later) of a 63-year-old man in 1968 |
| Reference(s) ： | 14891, 14734, 14600 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayer; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2 in 10 ml medium; split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA; maximal density at ca. 5-7 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80 hours |
| Harvest ： | cell harvest of ca. 3-4 x 106 cells/25 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype with 4% polyploidy - 82-88<4n>XXYY, -1, -4, -6, -9, -10, +12, -13, -18, +mar, der(17)t(9;17)(q11-12;q25) - apparent tetraploid derivate of original karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |